Helios BioSciences' know-how and 20 years expertise have enabled our company to optimize mRNA and protein expression analyses, using fast and cost-efficient techniques.
Helios BioSciences caters from experimental scheme design to result interpretation and ready-to-publish pictures
Tissue and Cell Expression Helios BioSciences provides you with its Tissue and Cell Expression (TCE) technological platform. Our platform relies on robust techniques in :
In Situ Hybridisation (ISH)
ImmunoHistoChemistry (IHC)
to study mRNA and protein expressions on tissue sections.
TCE is best used just after the results of DNA microarrays. TCE makes it then possible to determine where these mRNAs are expressed and to relatively quantify their expressions.
TCE is therefore an attractive option to rt-PCR and can be used as a first choice following the DNA microarray results.
In Situ Hybridisation (ISH) Helios BioSciences offers you different investigation strategies depending on your questions and on the abundance of the mRNA to be detected. Our expertise focuses on :
The making of probes and their labelling
Non radioactive labelling : riboprobes (fast qualitative analysis)
Radioactive labelling : oligonucleotides (easier to produce, they make the quantitative analysis possible)
Analysis at cellular and / or at tissular levels
Helios BioSciences analyses the images obtained and provides you with a detailed report the results
ImmunoHistoChemistry (IHC)
IHC analysis is based on a recognized platform composed of the whole equipment being required to run routine histological and immunohistochemical techniques.